In normal (or starting mode), each boss will occasionally drop a Recipe for a crafted relic that can be used as a portal to a hard mode version of the corresponding boss.

List Of Bosses

Normal Mode Hard Mode
Level Area Boss Level Area Boss
10 Worm Pit Ancient Worm 35 Ancient Lair Ancient Evil Electro Worm
20 Dragon's Lair Dragon Queen 35 Ancient Glacier Arctic Dragon Countess
30 World's End Hollow King 35 Ancient Refuge King of Light
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